AN IMPROVED MICRO PROPAGATION SYSTEM FOR SUCCESSFUL TRANSFORMATION OF CAULIFLOWER (BRASSICA OLERACEA VAR. BOTRYTIS)
نویسندگان
چکیده
منابع مشابه
Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica Oleracea var Botrytis)
Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appear...
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Serine transhydroxymethylase (EC 2.1.2.1) has been purified 46-fold from cauliflower (Brassica oleracea var. botrytis L.). The enzyme was completely dependent on the presence of tetrahydrofolic acid for the conversion of serine to glycine. The addition of pyridoxal phosphate gave a large increase in the reaction rate. A double pH optimum was observed with maxima at 7.5 and 9.5. The enzyme is sp...
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A rapid, simple and an efficient method for the determination of indoxacarb in cauliflower and soil samples was developed and validated using QuEChERS technique (Quick, Easy, Cheap, Effective, Rugged and Safe). Recoveries at four different spiking concentrations of 0.01, 0.05, 0.1 and 0.2 mg·kg ranged from 87% to 96% were achieved with good repeatability and RSD of 1% 6%. The average initial de...
متن کاملNuclear and plastid transformation of Brassica oleracea var. botrytis (cauliflower) using PEG-mediated uptake of DNA into protoplasts
Most plastid transformation studies with tobacco, and all reports for other species (except tomato [G.D. Nugent, M. ten Have, A. van der Gulik, P.J. Dix, B.A. Uijtewaal, A.P. Mordhorst, Plastid transformants of tomato selected using mutations affecting ribosome structure. Plant Cell Rep. 24 (2005) 341–349]), have used biolistics for plastid transformation. However, nuclear transformation via bi...
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In agricultural species that are sexually propagated or whose marketable organ is a reproductive structure, management of the flowering process is critical. Inflorescence development in cauliflower is particularly complex, presenting unique challenges for those seeking to predict and manage flowering time. In this study, an integrated physiological and molecular approach was used to clarify the...
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ژورنال
عنوان ژورنال: Acta Horticulturae
سال: 2012
ISSN: 0567-7572,2406-6168
DOI: 10.17660/actahortic.2012.961.19